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Head and Neck Surgery

(160) Effect of Photobiomodulation in Sonic-hedgehog Pathway Inhibited Rat Submandibular Gland

Monday, October 2, 2023
2:45 PM – 3:45 PM East Coast USA Time

    Presenting Author(s)

  • Yoon-Ah Kim, MD

    Resident
    Dankook University Hospital
    Cheonan, Ch'ungch'ong-namdo, Republic of Korea

    • Co-Author(s)

  • Phil-sang Chung, n/a

    Professor and Chairman
    Dankook University School of Medicine
    Chenan, Ch'ungch'ong-namdo, KOR

    • Senior Author(s)

  • SW

    Seung-Hoon Woo, n/a

    Dankook University Hospital, USA

Disclosure(s):
Yoon-Ah Kim, n/a: No relevant relationships to disclose.


Introduction: This study aims to verify whether Photobiomodulation(PBM), especially 850nm High power LED can restore the damaged structure and function of submandibular gland(SMG) by activating Sonic-hedgehog pathway(SHH) by using SHH-inhibited rat SMG model.

Methods: 12 male rats Spraggae Dawley (SD) Sprague Dawley (SD) were used in the animal experiment (120-140 g, 5 weeks old). We gavaged Vismodegib (a SHH inhibitor, 10mg/kg) for three weeks to decrease SHH. We divided the rats into three experimental groups and a control group and irradiation was proceeded with 850 nm high power LEDs at 60, 120, and 180 J/cm2 for 6 days(day 15 to 20). Western blot and immunofluorescence staining were used to observe the expression of SHH-related gene proteins (Shh, Ptch1, Smo, Gli1) and associated markers (AQP5, Amylase, Krt5). The SMG structure was examined using H&E staining.

Results: Compared to the control group, high power LED irradiation groups showed increases in the expression of SHH-related gene protein. Shh increased at 60J/cm2 (p < 0.05) and 120J/cm2 (p < 0.01). Smo increased at 120J/cm2 and 180J/cm2 (p < 0.05). Gli1 increased at 120J/cm2 (p < 0.05). The acinar cell marker AQP5 increased in all irradiation groups(p < 0.05). All irradiation groups had more stained AQP5 and Krt5(myoepithelial marker). With all of the irradiated groups, the structure of SMG was also restored on H&E staining.

Conclusions: This study found that 850nm High power LED can increase the expression of SHH-related gene proteins(Shh, Smo, Gli1) and associated functional markers(AQP5, Krt5). Also, LED irradiation restored the structure of SMG.
The results of this study may suggests that photobiomodulation can restore the damaged structure and function of SMG through activating SHH.