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Head and Neck Surgery

(168) Efficacy of Photobiomodulation on Geniculate Ganglion Neurons in Vitro

Monday, October 2, 2023
2:45 PM – 3:45 PM East Coast USA Time

    Presenting Author(s)

  • Hyoyeon Kim, MD

    Resident
    Dankook University School of Medicine
    Cheonan, Ch'ungch'ong-namdo, Republic of Korea

    • Co-Author(s)

  • Phil-sang Chung, n/a

    Professor and Chairman
    Dankook University School of Medicine
    Chenan, Ch'ungch'ong-namdo, KOR

    • Senior Author(s)

  • SW

    Seung Hoon Woo, PhD

    Dankook University School of Medicine
    Cheonan, KOR

Disclosure(s):
Hyoyeon Kim, MD: No relevant relationships to disclose.


Introduction: This study was designed to investigate the influence of photobiomodulation(PBM) on geniculate ganglion cell by measuring cell viability using MTT(Tetrazolium salt) assay, ELISA, and ADT/ATP ratio assay.

Methods: Geniculate ganglion neuronal cells were isolated from postnatal day 1 rats. GGNs were enzymatically segregated with papain. The isolated geniculate ganglion neuron cells were characterized by flow cytometry using surface markers. The PBM source, continuous wave type of 850 nm light emitting diode, was treated for 0, 1, 5, 10J on day 3 and MTT assay was done at post treatment day 1, 3, and 7. ADP/ATP ratio assay was conducted on post PBM day 7. The statistical analyses were performed using GraphPad Prism software. The differences between the treatment groups and control groups were calculated by one way ANOVA.

Results: All treatment groups (PBM 1, 5, 10J) showed increase of cell viability compared to the control group (0J). (p < 0.05). Also, it can be concluded that there is a direct positive correlation between the amount of given PBM energy and cell viability or ATP production level. (p < 0.05)

Conclusions: This study supports potential effect of PBM therapy by acceleration of oxygenation and ATP production, which can promote the regeneration of damaged geniculate ganglion neuronal cells. However, further studies and investigations are needed on the pathophysiology of taste alteration pathway. In addition, more investigations are needed to settle a gold standard protocol of PBM therapy.