(241) Ideal Culture Condition of Nasal Stem Cells in Human Nose

Introduction: Human Nasal Inferior Turbinate Derived Stem (hNTSCs) have been considered as an potent and usefulsource for regenerative medicine. To most effectively mimic the native environment of inferior turbinate could be very effectiveto hNTSCs biology. Thus the purpose of this study was to evaluate partial pressure of oxygen (ppO2) and temperature ininferior turbinate.

Methods: Ten patients were enrolled who underwent endoscopic endonasal transsphenoidal skull base tumor surgerybetween January 2014 and December 2015. The commercially available OxyLab pO2 monitor gauges the ppO2 andtemperature using a fluorescence quenching technique. Also, hNTSCs were isolated from 10 patients and cultivated underhypercapnic condition (5, 10, and 15%) to mimic hypoxic intranasal conditions.

Results: The measured oxygen concentration in submucosa tissue was higher than that at the surface of the inferiorturbinate and the temperature in submucosa tissue was higher than the value at the surface of inferior turbinate. The patternsof proliferation were significantly different according to hypercapnic cultivation conditions and there were statistically significantdecreased proliferation rates after the exposure of higher CO2 over a period of 5 days

Conclusions: Intranasal turbinate tissue showed the hypoxia state in concordance with the result of theother tissues or organs. However, indirectly induced hypoxia influenced the influence on the hNTSCs proliferation negatively.Further study is needed to mimic the real hypoxic state, but our results could be used to optimize the culture environment ofhNTSCs, thereby producing the stem cells for regenerative therapies.